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pHelix_001
Backbone
pLacIq
RBS_B0034
mCherry_opt
T_B0015
CmR_marker
ori_pUC
pHelix_002
pHelix_003
genomic_edits
BioBrick Palette
▶Promoter
▸RBS
▰CDS
■Terminator
○Origin
◆Marker
▷Inducible
▦Fusion
pHelix_001 CONSTRUCT · ID:HX-001-7C3F
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0 bp
7200 bp
Circuit A — Fluorescent Reporter
CIRCULAR
GC: 54.2%
Tm: 78.3°C
Length: 7,200 bp
pLacIq
R
mCherry_opt
T
CmR
Circuit B — Inducible Cascade
LINEAR
GC: 51.8%
Tm: 72.1°C
Length: 3,840 bp
pTet
R
tetR
T
pLac
R
GFP_mut3
T
Circuit C — CRISPR Guide Array
CIRCULAR
GC: 48.3%
Tm: 69.7°C
Length: 4,512 bp
ori
pJ23119
R
dCas9
T
Inspector
Simulation
Checks
Selected Part
NamemCherry_opt
TypeCDS
Length711 bp
Position252 – 963
StrandForward (+)
GC Content58.4%
Melting T84.2 °C
Properties
Display Name
Color Tag
Codon Optimization
Annotations
Registry IDBBa_J18912
Authorhelix-team
Version2.1.0
StatusVerified
Restriction Sites
EcoRI1 site
BamHI0 sites
XhoI2 sites
NdeI0 sites
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